Journal: EMBO Molecular Medicine
Article Title: SOX9-dependent fibrosis drives renal function in nephronophthisis
doi: 10.1038/s44321-025-00233-3
Figure Lengend Snippet: ( A ) Cystic index represented as the percentage of cystic area per kidney section from 1-, 3-, 7-, and 10-month-old Fbxw7 f/f and Cdh16Cre;Fbxw7 f/f mice. Each data point represents one animal (n ≥ 3). Statistical analysis was performed using two-way ANOVA and is presented as the mean ± SEM. ( B ) Representative images of LTA+ and mG+ cystic tubules in Cdh16Cre;ROSA mT/mG ;Fbxw7 f/f (middle and right panels) and Cdh16Cre;ROSA mT/mG (left panel) mice. White arrows show cystic mG+ tubules that are LTA + . Nuclei are stained with DAPI (blue). Scale bar: 50 µm. ( C–J ) Representative images of immunofluorescence staining for ( C , D ) proximal tubule marker, Lotus tetragonolobus agglutinin (LTA), ( E , F ) thick ascending limb of the loop of Henle marker, NKCC2, and ( G , H ) collecting duct marker, Dolichos biflorus agglutinin (DBA) from 3- and 7-month-old kidneys of Fbxw7 f/f and Cdh16Cre;Fbxw7 f/f mice. White arrows show dilated or cystic tubular segments of the kidney that are positive for ( C , D ) LTA (green), ( E , F ) NKCC2 (pink), and ( G , H ) DBA (red). Nuclei are stained with DAPI (blue). Scale bar: 50 µm. ( I , J ) Pie chart displaying the percentage of cystic tubules distributed across different kidney segments from 3- and 7-month-old Fbxw7 f/f and Cdh16Cre;Fbxw7 f/f mice ( n ≥ 3).
Article Snippet: Fluorescein (1:1000; Vector Laboratories FL-1321) or Cy5 (1:1000; USBIO 518482) conjugated Lotus Tetragonolobus Lectin (LTA) and Rhodamine conjugated Dolichos Biflorus Agglutinin (DBA) (1:1000; Vector Laboratories RL-1032-2), was added to co-stain proximal tubules and collecting ducts, respectively, in the secondary antibody solution wherever needed.
Techniques: Staining, Immunofluorescence, Marker
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